TF-1 cells were transformed to GM-CSF independence by expression of the N676S mutant of Flt3-ITD. time points. The producing curves were match to the Michaelis-Menten equation using GraphPad Prism v7.04, and the resulting Km ideals are shown in the Table at ideal. B) Dedication of intrinsic kinase activity. Each kinase … Continue reading TF-1 cells were transformed to GM-CSF independence by expression of the N676S mutant of Flt3-ITD